Answer and Explanation:
The steps for construction of the expression plasma
- STEP 1 : Introducing Notl and Hindlll confinement sites at the ends of the gene of interest by means of PCR
- STEP 2 :Digest the plasmid and DNA encoding gene with the Notl and Hindol confinement endonucleuses.
- STEP 3 : Ligate the cut plasmid and DNA fragment
- STEP 4 : Change the plasmid into E.coli for expression
- STEP 5 : Clean the protein by utilizing a column of immobilized

1. The answer is Density
2. Two ways to make peace with your body are to create a positive mental outlook and to give yourself credit
3.When on a weight-gain diet, untrained men tend to initially gain about 2 pounds of muscle per month, and 20 pounds or more per year. This is because there is more area in the body available for muscle growth that's why muscle fibers are easily developed and recruited to perform muscle contractions.
4. <span>figure out where in your day you could eat more food.
5. amino acids
6. weight
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Answer: If you want to reduced your energy intake then you have to carefully controlled your diet with such food as;
-Breakfast cereal
-white bread
-paste
-rice
-yogurts and other food with added sugar .
As well as drinks like Alcohol or coffee
That's all Hope this answers are helpful.
Answer:
The Force vs Speed F-V, relationship assumes that at a given velocity, the muscles (load) are generating the maximum force possible.
Explanation:
This is because, as the external load (i.e. mass) increases, the maximal velocity that such load achieves decreases. The load-velocity relationship assumes that the movement velocity is the maximum possible for the given load.