Probes that bind under very stringent hybridization conditions resulting in the differential binding over one nucleotide are cal
led ________. Probes that bind under very stringent hybridization conditions resulting in the differential binding over one nucleotide are called ________. short, variable repeats VNTRs microsatellites allele-specific oligonucleotides (ASOs) generation-specific probes
An allele-specific oligonucleotide (ASO) is a piece of synthetic DNA that is short and usually a complementary to the sequence of a target DNA, It is an oligonucleotide of 15–21 nucleotide bases in length.
It acts as a probe in southern blot assay or simpler Dot blot assay to identify the presence of a target.
It is an important research tool in genetic testing, forensics, and Molecular Biology.
The probes can detect a difference of as little as 1 base in the sequence targeted.
They get runoff into water sources which can be injested by fish which can be injested by birds or any animal who eats the fish or drinks the water including us!