Step 1: Lysis
In this step, the cell and the nucleus are broken open to release the DNA inside and there are two ways to do this. First, mechanical disruption breaks open the cells. This can be done with a tissue homogenizer (like a small blender), with a mortar and pestle, or by cutting the tissue into small pieces. Mechanical disruption is particularly important when using plant cells because they have a tough cell wall. Second, lysis uses detergents and enzymes such as Proteinase K to free the DNA and dissolve cellular proteins.
Step 2: Precipitation
When you complete the lysis step, the DNA has been freed from the nucleus, but it is now mixed with mashed up cell parts. Precipitation separates DNA from this cellular debris. First, Na + ions (sodium) neutralize the negative charges on the DNA molecules, which makes them more stable and less water-soluble. Next, alcohol (such as ethanol or isopropanol) is added and causes the DNA to precipitate out of the aqueous solution because it is not soluble in alcohol.
Step 3: Purification
Now that DNA has been separated from the aqueous phase, it can be rinsed with alcohol to remove any remaining unwanted material and cellular debris. At this point, the purified DNA is usually re-dissolved in water for easy handling and storage.
Section 2 of Article 2 lays out the powers of the presidency, establishing that the president serves as the commander-in-chief of the military, among many other roles.
<u>
Explanation:
</u>
Constitution of America establishes various powers and execution of power for the president. The constitution has given certain power to president who can have control over the federal government and implement the federal laws.
In US Constitution Section 2 of article 2 which clearly states that the president will be the commander in chief of the military and he has been assigned power to initiate action wherever required. This particular section 2 of Article 2 has given the power to the president to be invoked in case of emergency.
To prevent falls and protect workers, OSHA requires guardrails or personal fall arrest systems for those working on scaffold platforms 6 feet or higher.
OSHA calls for that fall safety to be supplied at elevations of 4 feet in standard industry offices, 5 toes in shipyards, six ft within the construction industry, and eight ft in long shoring operations.
OSHA usually calls for fall safety to be provided at four feet in the popular industry, 5 ft in maritime, and 6 ft in production. But, no matter the autumn distance, fall safety needs to be furnished while running over risky equipment and equipment.
Learn more about OSHA here brainly.com/question/1264446
#SPJ4
Answer:
The United States military uses the 5.56x45 projectile in a standard grain of 62 g. The
velocity of that round at 100 yards is 2714 fps. Most countries that oppose the United
States prefer the larger projectile, the 7.62x39 in a standard grain of 123 g. That round
has a velocity of 2008 fps Which projectile is more powerful (does the most damage)?
Explanation:
Answer: An act of piracy must meet very specific criteria to be considered an act of piracy according to the International Maritime Bureau.
Explanation: Among those criteria are: The pirates must board another vessel; The pirates must intend to steal or commit another crime.
This is not Plagiarized and can be directly copied and pasted to whatever assignment your working on.