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Ulleksa [173]
3 years ago
13

What unique things would separate you from other applicants applying for this money?

English
1 answer:
Vinvika [58]3 years ago
8 0

Answer:

I work for a quick lube

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B

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Narration means the art of storytelling, and the purpose of narrative writing is to tell stories. Any time you tell a story to a friend or family member about an event or incident in your day, you engage in a form of narration. In addition, a narrative can be factual or fictional. A factual story is one that is based on, and tries to be faithful to, actual events as they unfolded in real life. A fictional story is a made-up, or imagined, story; the writer of a fictional story can create characters and events as he or she sees fit.

The big distinction between factual and fictional narratives is based on a writer’s purpose. The writers of factual stories try to recount events as they actually happened, but writers of fictional stories can depart from real people and events because the writers’ intents are not to retell a real-life event. Biographies and memoirs are examples of factual stories, whereas novels and short stories are examples of fictional stories.

Know Your Purpose

Because the line between fact and fiction can often blur, it is helpful to understand what your purpose is from the beginning. Is it important that you recount history, either your own or someone else’s? Or does your interest lie in reshaping the world in your own image—either how you would like to see it or how you imagine it could be? Your answers will go a long way in shaping the stories you tell.

Ultimately, whether the story is fact or fiction, narrative writing tries to relay a series of events in an emotionally engaging way. You want your audience to be moved by your story, which could mean through laughter, sympathy, fear, anger, and so on. The more clearly you tell your story, the more emotionally engaged your audience is likely to be.

The Structure of a Narrative Essay

Major narrative events are most often conveyed in chronological order, the order in which events unfold from first to last. Stories typically have a beginning, a middle, and an end, and these events are typically organized by time. Certain transitional words and phrases aid in keeping the reader oriented in the sequencing of a story. Some of these phrases are listed here

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No matter how you look at it, we are in good hands<br>​
Alex

yes that is right!!!!!!

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What is an events chain concept map
Nostrana [21]

Recombinant DNA (rDNA) = Vector DNA + Passenger DNA

In genetic engineering, a foreign gene is inserted into an organism's genome to alter its phenotype or to get useful products beneficial to humans.

STEP 1
In order to get the DNA, which is enclosed within the cell membranes, we have to break open the cell to release the DNA. For this, enzymes are used such as lysozyme (for bacteria), chitinase (for fungus) and cellulase (for plant cells).
This results in the release of DNA along with the other macromolecules like RNA, proteins (histones).
Now, in order to purify and separate the DNA, ribonuclease and protease are added to digest the RNA and Proteins to separate the DNA.
Next, the separated DNA is precipitated in the suspension by adding chilled ethanol and then it is separated out by spooling or winding over a fine appliance.

STEP 2
Now we have obtained the DNA. Next step is to cut the DNA by using the Restriction enzyme to get the desirable genes. The same restriction enzyme is used for both the vector DNA and the passenger DNA to produce the complementary cut parts (sticky ends). The cut out DNA fragments can be amplified to billions of times by PCR (Polymerase Chain Reaction). Agarose Gel Electrophoresis is done to separate the DNA fragments.
Vector DNA is ligated with Passenger DNA by the enzyme ligase to form the rDNA.

STEP 3
Now this rDNA is inserted into the bacterial cells by gene gun or bolistic gun, or by making them competent to take up the rDNA by treating them with divalent cations.

STEP 4
Bacterials cells are cultured in large containers called BioReactors where large amount of culture (100-1000 litres) can be processed at the same time.

STEP 5
Finally the product is obtained, purified and separated called Downstreaming Process.
The product is also formulated with the preservatives before marketing.

Example:- In 1983, Eli Lilly, an American Company produced the Genetically Engineered Insulin called 'Humulin'
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