The "scissors" of the molecular biology are: DNA Restriction enzymes.
A restriction enzyme is a protein capable of cleaving a DNA fragment at a characteristic nucleotide sequence called a restriction site. Each restriction enzyme thus recognizes a specific site. Several hundred restriction enzymes are currently known.
Naturally present in a large number of species of bacteria, these enzymes have become important tools in genetic engineering.
The "glue" of the molecular biology are: DNA ligase
In molecular biology, DNA ligases are ligase-class enzymes that catalyze the formation of a phosphodiester bond between two segments of DNA. DNA ligases are involved in several essential cellular processes of DNA metabolism: in DNA replication, suture of Okazaki fragments, and in DNA repair and homologous recombination.
The use of these tools in molecular biology: Cloning
Molecular cloning is one of the bases of genetic engineering. It consists of inserting a DNA fragment (called insert) in an appropriate vector such as a plasmid for example. The new plasmid thus created will then be introduced into a host cell, generally the Escherichia coli bacterium. This will then be selected and multiplied to obtain a large amount of the plasmid of interest. Cloning a gene involves inserting it into a plasmid. A clone will be the bacterial transformant that contains this particular plasmid. In this case we speak of clone because all the individuals of the bacterial colony are genetically identical. Molecular cloning is thus different from reproductive cloning (creating an individual genetically identical to another but of a different age) or therapeutic cloning (making tissues from stem cells to perform transplants compatible with the recipient).
Molecular cloning requires restriction enzymes capable of cleaving the DNA, and DNA ligase capable of re-gluing the DNA fragments. Ligase was isolated for the first time from T4 bacteriophage. This enzyme is involved in the repair and replication of DNA. It can bind DNA fragments with compatible sticky ends. At higher concentration, this enzyme is also able to bind two ends of DNA as shown here. T4 DNA ligase works using ATP and Mg ++. It has an activity optimum of 16 ° C, but remains active at room temperature.
Answer:
Golgi body ^_^
Golgi Bodies help process protein and sort out the cellular proteins for delivery inside or outside of the cell!
Answer:
Earth, both on land and in water. Wherever they
live, plants provide food and oxygen to the
creatures that live nearby – including humans!
In this activity we will examine both a
terrestrial (land) plant and an aquatic (water)
plant. They have many things in common, but the
ways they get air, food and water change along
with the environments in which they live.
Explanation:
Capture myopathy is a heart condition in animals specifically in tamarins. Dr Natterson-Horowitz studied the condition and discovered that it is similar to cardiomyopathy which is a heart condition in humans. She also studies some conditions that are parallel to both humans and animals.<span />
Answer:
Human activities affect the flow of energy and matter in an ecosystem and alter the energy balance in ecosystems through the unsustainable nature of what they do. The energy flow is affected in several ways as a result of pollution, overpopulation, deforestation, burning fossil fuels, etc. Such changes have stimulated soil erosion, climate change, causing water unfit to consume, poor quality of air and so on. When humans cause a change in the energy balance, they impact the ability of the ecosystem to respond and adapt to changes in the environment. It is like getting a cut, but it never heals and grows bigger instead.
