<span>Benzodiazepines , which are included in a class of drugs called hypnotics; some types of benzodiazepines include clonazepam (Klonopin), diazepam (Valium), temazepam (Restoril), estazolam, alprazolam (Xanax), and lorazepam (Ativan). These drugs may be used to treat parasomnias. Occasionally, they are also used to treat bruxism (teeth grinding) and short-term insomnia.</span>
Answer:
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The following changes influence the sensitivity of PCR primers for the target DNA sequence in a PCR reaction Increase.
The most effective way to improve specificity is to design primers that are nucleotides in length with a high annealing temperature of Tm. This is especially important when using genomic DNA or cDNA libraries as PCR templates. A pair of primers hybridizes with the sample DNA and defines the region to be amplified. The result is millions of copies in a very short time.
Primers are also used for DNA sequencing and other experimental procedures. A PCR primer refers to a short piece of single-stranded DNA used in a PCR reaction, and a sequencing primer refers to a short sequence of nucleotides used to initiate DNA synthesis in a sequencing reaction. Use the lowest possible concentration if necessary.
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