Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
Mass doesn't change no matter where you are or where you go.
Explanation:
Mass is what you or an object consist of. You cant change mass
The term "pneumocephalus" refers to the condition in which air is present in the cranial cavity.
What happens in pneumocephalus?
- Increased intracranial pressure causes tension pneumocephalus, a neurosurgical emergency that can cause headaches, seizures, loss of consciousness, and even death.
- Clinically separating the two entities is difficult but essential.
- Tension pneumocephalus following skull base surgery was found to be a rare phenomenon with just eleven cases described, according to a survey of the literature on PubMed/MEDLINE.
- After treatment, the majority of patients experienced full symptom relief.
- Following skull base surgery, tension pneumocephalus is a potential complication that clinicians should be aware of.
- An understanding of imaging characteristics and a high level of suspicion are necessary for accurate diagnosis. Rapid management is essential to avoid potentially disastrous effects.
Learn more about human skull with the help of the given link:
brainly.com/question/4537715
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Step One - Obtain a clean microscope slide.
Step Two - Place a drop of liquid on the slide. This is the “wet” part of the wet mount. The liquid used depends on the type of cell being viewed:
If examining a plant cell, tap water can be used.
If examining an animal cell, physiological saline (or contact lens solution) must be used, because if plain water is used, the cell will explode from osmotic pressure. Unlike plant cells and bacteria, animal cells have no cell wall to structurally support them.
Step Three - Obtain the specimen to be used. Some introductory biology classics for viewing include:
Skin of an onion bulb: In order to view the cells, a very thin layer of skin must be obtained. Take a single layer of onion and bend it towards the shiny side. After it snaps, pull gently, and a transparent layer of skin, similar to Scotch tape, will appear.
Elodea leaf: Elodea leaves are two cell layers thick. The cells in one layer are smaller than the cells in the other, so elodea leaves can be used to better understand a microscope's depth of field.
Cheek cells: Human epithelial cells can be obtained by gently rubbing a toothpick on the inside of the mouth, and then swirling the toothpick in the physiological saline on the slide.
Pond water: Obtaining some water from a pond makes wet mount preparation a breeze, since the water and the specimens are both included.
Hope this helps
The right option is; C. cinder cone, a pyroclastic flow with no explosion
Basaltic magma is a fluid rock material produced from basalt and it has high amounts of iron and magnesium, and low amounts of silica. Basaltic magma is formed within the earth when a part of the earth's mantle is melted. Basaltic magma produces three different types of lava or volcanic deposits which are scoria, ash or cinder, and lava flows.
