The EMB (Eosin Methylene Blue) agar is a selective and differential agar medium. It contains sucrose and lactose as fermentable substrates together with Eosin Y and methylene blue dyes which in combination gives the agar its characteristic color when prepared and serves as a pH indicator as well as an inhibitor of growth of Gram-positive bacteria. Hence, it is primarily used to isolate Gram-negative fecal coliforms while some positive fecal coliforms such as Staphylococci are also able to grow.
These coliforms are of two types:
- Lactose or sucrose fermenting coliforms form metallic sheens on the agar as a result of acid production and the response of the indicator to the increased acidity.
- Non-lactose/sucrose fermenters are only able to produce acid by protein deamination. Hence they either form colorless or pinkish colonies on the agar
On the other hand, Mannitol salt agar is a selective and differential agar medium that is primarily used to isolate Staphylococcal bacteria. The presence of sodium chloride in the medium makes it a partial or complete inhibitor of other bacteria.
Hence, an unknown bacterium that forms colorless colonies on EMB will either be a non-lactose fermenting, Gram-negative coliform that will hardly grow on Mannitol salt agar, or a Gram-positive fecal Staphylococcus.
Staphylococci bacteria are of two types:
- Coagulase-positive will form yellow colonies on the Mannitol salt agar.
- Coagulase-negative will form red colonies on the Mannitol salt agar.
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Answer:
Viruses are like hijackers. They invade living, normal cells and use those cells to multiply and produce other viruses like themselves. This can kill, damage, or change the cells and make you sick. Different viruses attack certain cells in your body such as your liver, respiratory system, or blood.
Explanation:
Viruses tend to target specific tissues (cells) in the host.
For example, the influenza virus has a predilection for the respiratory tract, hepatitis viruses target the liver, polio virus targets the motor neurons of the spinal cord and rotavirus multiplies in the gut. Symptoms of a viral infection may be subtle and nonspecific or specific and suggestive of the causative agent.
Dengue virus, Ross river virus, measles and rubella infections are associated with fever and a widespread red rash, chicken pox and herpes simplex viruses are associated with blistering, often localized, rashes; and hepatitis viruses cause liver damage and jaundice.
Bacteria tend to be less tissue-specific and non-discriminatory than viruses and can cause a variety of infections once they have invaded the host.
These bacterial infections are often manifested by the presence of pus wherever the bacteria settle, and systemic symptoms such as fevers, chills, pain, swelling and loss of function occur when bacteria invade and multiply.
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