It was primarily the "b. formation of the Vietcong in Vietnam" that led the Truman administration to expand the containment doctrine to include Asia, since this was seen as a threat to democratic principles in the west as well.
One of the two earliest civilization of the Middle East are Ancient Egyptians and Mesopotamian.
It would interfere with their trade alliances.
Communism leads to the government taking control of resources and industry. If it spread into areas the US did business, they could lose the ability to control resources and trade. This would eventually impact our political power and any military advantages the US had in Europe.
Boiling ensured that there were no living organisms in the broth at the beginning of the experiment.