Tonic Pressure is the pressure exerted by water moving during osmosis. Tonic pressure occurs when two solutions are separated by a semi-permeable membrane, allowing for the solution to go through.
Answer:
It's important to not touch the sides or the bottom of the test tube with the thermometer because touching it to the sides or bottom of the test tube fractures the glass and does not allow for correct temperature measurements.
A point mutation can indeed be a frameshift mutation