When you enumerate you need to put comas between all the items except the ones which are connected by "and" : as A is not correct as it does not have comas, and B is incorrect as it has comas where "and" is.
now, in a way both C and D are correct, but C is the usual accepted version, and D is an example of "Oxford coma" (where the coma is optional, to remain on the safe side, choose C).
It really depends on Charlie's co worker and the outcome will be on Charlie's personality
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you forgot to post the question but I will gladly help when if you comment the question
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the butterfly symbolism works as a representation of resurrection, change, renewal, hope, endurance, and courage to embrace the transformation to make life better.
In Day of the Butterfly by Alice Munro we have the theme of isolation, commitment, connection, betrayal, appearance and friendship.