The line from "The Nymph's Reply to the Shepherd" which does NOT express the concept of transience is the following one:
All these in me no means can move
The line above expresses the idea that none of the things described in the poem can lead the nymph to be the shepherd's love. Therefore, it has a sorrowful tone but doesn't express transience.
As for the other lines, they all carry the idea of time passing by, of it being ephemeral, that is, transient.
The author expresses transience by using figures of speech.
