Amoeba is a unicellular heterotrophs.It has no mouth but engulf food particles by spreading two of its pseudopodia and the food find its way into the food vacoule.The digested food is absorbed by the protoplasm by diffusion.
The most chloroplasts are found in the leaves due to the fact the chloroplast are not need in the roots as they are underground and have no light
Affinity chromatography is a method used to separate molecules with high affinity. This technique enables the purification of specific molecules ranging form nucleic acids, enzymes, antigens, antibodies, etc. In the example, the ligand will produce the receptor to be displaced from the beads and then eluted out.