Answer:
DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments
Explanation:
Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., <em>BamH</em>), or blunt ends, where the resulting strands are of the same length (e.g., <em>HaeIII</em>). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.
Exploring new sources of coal depositsAnswer:
Explanation:
sry if im wrong
Explanation:
the oxygen that fish breathe is not the oxygen in H2O.Instead,the fish are breathing O2(oxygen gas) that is dissolved in water. ...Plus water is a lot heavier and thicker than air,so it takes a lot more work to move it around. please mark me braneliest
Answer:
(a) When the enzyme phosphorylase is allowed to treat with the ATP and phosphorylase kinase, this helps in the activation of the more active form of the phosphorylase. This increase the process of the glycogen breakdown in the cell.
(b) PP1 ( protein phosphatase 1) removes the phosphate group from the substrate that results in the activation of the less active form of the enzyme. The process of glycogen breakdown in the cell will decrease.
(c) The hormone epinephrine causes the release of cAMP that increases the kinase activity. The active form of the enzyme predominates and increases the glycogen breakdown in the body.