Answer:
The correct answer is b.Amplify the gene using PCR. Insert the gene into a plasmid vector. Transform the vector into the bacteria.
Explanation:
If I have a very small amount of gene for a fluorescent protein than the first step is to amplify the gene so that appropriate protein can be produced. PCR is the instrument that is used to amplify the protein.
So after amplification of the gene, the plasmid vector will be used in which the gene will be inserted because the plasmid vector is used to insert this gene in host cells where protein will be expressed.
The final step will be to transform bacteria with recombinant plasmid so that plasmid can make its copy and express a fluorescent protein in bulk.
Answer:
3,2,1,4
Explanation:
as time goes on the water levels rise it expands the bay of the river
Answer:
d. more permeable to sodium ions.
Explanation:
The binding of Acetylcholine with the receptors at motor end plate facilitate membrane permeability to sodium ions therefore opening of voltage gated channels for sodium ions.
Sodium ions diffused into the synapse of post synaptic neuron at the neuromuscular junction.
If the stimulus is up to threshold levels; more chemical(neurotransmitter) gated sodium channels opens, and sodium ions move into the synapse to depolarize the membrane with reversal of charges to generate action potential.
The continuous binding of Acetycholine , leads to permanent opening of sodium ions,and over excitation from depolarization. Acetycholinesterase enzymes breakdown Acetycholine to choline and Acetyl Co -A. These are recycle back to Acetycholiine
