Part A
Plasmids are extracted from the bacteria
The plasmids are spliced at specific regions using restriction enzymes.
The cut plasmids are incubated with the novel gene and DNA ligase
Plasmids that will have incorporated the new gene are transformed back into the bacteria
Part B
The answer is transgenic organisms.
Answer:
Condensation, melting, evaporation, then it is either 1 or 2
Explanation:
Answer:
C.The added epitope disrupts the function of the tagged protein
Explanation:
When an additional sequence is tagged to a protein to use comercial antibodies, there are several reasons why this procedure wouldn't work as expected (note that we're assuming the protein is being expressed but it's not possible to detect it).
For example, the sequence of nucleotide added to codify for the tagged epitope are removed during the RNA processing. In that case, the protein would be expressed without the epitope, so it would be impossible to localize it with the antibodies.
Also, it could be that the new epitope is affecting some way the protein folding, making it not functional. This way, it would be degraded by the cell so it wouldn't be detected.
Another possibility is that the epitope doesn't affects the protein folding nor its function, but during the folding ends up in a conformation that makes it inaccesible for the antibody.
In summary, the way as the possible answers to this question are shown, the correct option seems to be C:<em> The added epitope disrupts the function of the target protein.</em>
There’s no diagraaaammmmm.
Answer:
C
Explanation:
Usually if 1 out of 4 children in a family have a different hair color, it is because of the recessive gene passed down from the mother or fathers genes.
