I think It purifies the soluble GFP in the supernatant by passing over the supernatant over an HIC columnin a highly salty buffer.
At the Earth's surface, rocks usually break quite quickly, but deeper in the crust, where temperatures and pressures are higher, rocks are more likely to deform plastically. Sudden stress, such as a hit with a hammer, is more likely to make a rock break. Stress applied over time often leads to plastic deformation. Hope this helps. ヅ
Food enters the stomach during the third stage of digestion, hope this helps.
The BRAIN. All of the senses send message to the Brain