When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
All of these snake populations would increase.
Answer:
<h2>D. Competitively dominant to other organisms in the ecosystem</h2>
A keystone species is an organism that helps define an entire ecosystem. Without its keystone species, the ecosystem would be dramatically different or cease to exist altogether.
<em>Hope that helps! :)</em>
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<em>-Aphrodite</em>
Explanation:
It’s number 4 I believe please give me Brainlest
Answer:
Topoisomerase
Explanation:
Topoisomerases are enzymes that produce changes in the topology of the DNA during replication, transcription, traduction, or reparation processes. They can cut one or both strands and in order to relieve torsional stresses in the supercoiled structure of DNA. With this, they help to maintain the chromosome's integrity. There are two types of topoisomerases: topoisomerase I (it cuts only one strand of DNA) and topoisomerase II (it is able to cut both strands of DNA).
