Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
umm is this a staement or a question
Explanation:
Answer:
It would have disastrous consequences on the viability of the individual
Explanation:
The genome can be defined as the genetic material of an organism, which is composed of DNA molecules named chromosomes whose building blocks are four different nucleotides: Adenine, Thymine, Cysotcine and Guanine. The order of these nucleotides specifies the information contained in the chromosomes and, ultimately, in the genome. The genes, which are recognized to be the basic units of inheritance, are genetic sequences composed of ordered nucleotides along the chromosomes. During transcription, a gene encodes different RNA codons (for example, start and stop codons) which are specified by the order of the nucleotide sequence. Finally, the order of the RNA codons will be critical to specify the polypeptide chain during the translation from RNA to protein.
AB- is the blood type that is least common and 0+ is the most common
Am certain that Creatine phosphate functions in the muscle by storing energy that will be transferred to ADP to resynthesize ATP. All muscle cells have a little ATP within them that they can use immediately, however, only enough to last for about 3 seconds. Therefore, all muscle cells contain a high-energy compound called creatine phosphate which is broken down to make more ATP quickly.
