While the absorbance at 420 nm is related to the amount of o-nitrophenol produced, the absorbance at 600 nm is proportional to cell density, which aids in standardizing our estimations of enzyme activity.
<h3>Describe absorbance.</h3>
The amount of light absorbed by a solution is measured by its absorbance (A), often referred to as optical density (OD). The amount of light that may flow through a solution is called its transmittance.
<h3>How is the activity of beta-galactosidase determined?</h3>
The colorless ONPG substrate is changed by beta-Galactosidase into galactose and the chromophore o-nitrophenol, which results in a vivid yellow solution. The amount of substrate transformed at 420 nm can be calculated by measuring the solution's beta-galactosidase activity using a spectrophotometer or a microplate reader.
<h3>What is measured by the beta-galactosidase assay?</h3>
The -Gal Assay Kit gives users the tools they need to swiftly assess the amounts of active beta-galactosidase expressed in cells that have been transfected with plasmids encoding the lacZ gene.
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With Freezing.... the substance energy level decreases.
Freezing is endothermic and as such energy is lost to the environment
from the substance thus decreasing the energy level.
Answer:
A)
global temperature increasing as CO2 concentration increases
Explanation:
Global warming is the progressive increase in the temperature of the earth's atmosphere. Global warming is caused by an increase in the concentration of greenhouse gases e.g CO2, chlorofluorocarbons etc. These greenhouse gases, in which carbondioxide is an important example, are capable of trapping heat in order to keep the earth warm.
Howeever, as the concentration of CO2 and other greenhouse gases increase in the atmosphere, the earth's atmospheric temperature also increases above normal, causing Global warming.
In this case, the science teacher's graph will show that global temperature is increasing as CO2 concentration increases.
DNA contained within chromosome, chromosome in the cell nucleus
