In my opinion mixed layers would be the best choice
Explanation:
In the early days of development, in an embryo, the cells are pluripotent and can differentiate into any kind of cell of the organism. As the embryo develops, autocrine and paracrine signaling between the cells of the embryo causes the cells to migrate to particular regions of the embryo and begin differentiating into respective parts of the organism. This signaling causes differential silencing of particular genes of the cells depending on the type of cells they will differentiate into. As the embryo develops into a fully grown organism, these cells lose potency (except for stem cells) and become fully differentiated into respective limbs or organs of the organism.
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Answer:
a. Oxygen performs an essential role in the mentioned microbial cell in a manner that it takes part in the procedure of glycolysis, Krebs cycle, and electron transport chain, which eventually assists in the production of energy from food substrates and this generation of energy helps the cell to survive.
In the existence of oxygen, sugar gets dissociated through glycolysis to generate pyruvate, which again in the existence of oxygen is transformed into acetyl CoA. This moves into the Krebs cycle and gets dissociated to water and carbon dioxide generating ATP through ETC. This generation of ATP helps the cell to survive.
In low oxygen surrounding or in the absence of oxygen, some of the aerobic microbes can switch their respiratory pathway and carry on the process of fermentation and anaerobic respiration to produce energy and thrive. However, the mentioned microbial cell, which when it comes in contact with the low oxygen environment cannot carry out fermentation process and would die eventually.
b. This organism can be classified as obligate aerobes as they always need oxygen and do not possess the tendency to carry out the process of anaerobic respiration or fermentation under the absence of oxygenic environment.