The most efficient procedure that scientist can use to obtain more
recombinant dna for a particular trait is Clone the dna in
a biological vector. This method will give more assurance that they
will get the traits that they wanted form the subjects.
Answer:
Explanation:
What Mendel wanted to happen was to obtain middle-sized plants. What actually happened was that Mendel got tall plants. Due to this result, Mendel called Reccesive to the genes thatdetermined the short offspring, and Dominant to the genes that determined the tall offspring.
Gross motor skills involve large muscles in the arms legs and torso. The general guidelines for gross motor development for children 4 years of age include running, jumping and climbing well and control their direction. They can hop on one foot and they start to skip at this age. At this stage, they are also able to ride a tricycle and reliably catch a ball.
The answer to your question is 4.
Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
Well it’s pretty easy 7 - 15 is 8
