Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
The nucleus at the central part of the cheek cell contains DNA. When a drop of methylene blue is introduced, the nucleus is stained, which makes it stand out and be clearly seen under the microscope.
Copied
<span>Light dependent reactions require an actual source of light, and this is from the sun, for energy to carry out the first stage of photosynthesis. They need solar energy to split a water molecule in order to obtain electrons from hydrogen to be used in fueling photosynthesis. Light independent reactions do not require a light source for energy but rather they use energy that was created by the light dependent reactions to complete the process of photosynthesis in which different carbohydrates for the plant are made.</span>
Answer:
kingdom
Explanation:
kingdom is the broadest taxonomical classification
Answer:
In the English language, the word scent is used specifically for a kind of smell that is pleasant or has fragrance.
On the other hand, in the English language, the word smell is used to describe any kind of odour. The odour might be pleasant or not pleasant. It can be good as well as bad. Smell refers to any kind of smell which our olfactory receptors can determine.
