Answer:
<h2>Mitochondria was affected by the poison cellular respiration </h2>
<h3>Hope it helps you </h3>
Only 7% of the earth’s land surface but contains over 50% of the worlds wildlife
Option(a) Monera is the correct answer.
In the obsolete five-kingdom system, prokaryotes were included within Monera group.
<h3>What are prokaryotes?</h3>
- Prokaryotes are creatures without a nucleus or other organelles in their cells.
- Bacteria and archaea, two separate groupings of prokaryotes with allegedly independent evolutionary histories, are separated into.
- The majority of prokaryotes are tiny, single-celled creatures with a straightforward structure.
<h3>The definition of Monera:</h3>
- the prokaryotic kingdom that includes bacteria, reproduces primarily asexually by budding or fission, and obtains its nutrition through photosynthesis or chemosynthesis.
<h3>What are five Monera examples?</h3>
- <u>Cyanobacteria</u>, which are blue-green algae
- <u>Streptococcus</u>, which are cocci-shaped bacteria
- <u> E</u>. <u>Coli</u>
- <u>Vibrio</u> <u>cholerae</u> (cholera)
- <u>Treponema</u> <u>pallidum</u> (syphilis)
- other bacteria with a spiral form.
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Answer:
No
Explanation:
Organisms cannot willingly change their mutations as these things occur by chance and within the genetic code which the organisms have no direct power over.
1. Dysentery caused by <em>Shigella dysenteriae</em><em> </em>is isolated using MacConkey Lactose agar and Xylose Lysine agar
2. Antibiotic associated diarrhea caused by <em>Clostridium difficile</em> (detection of toxin) is isolated using <em>Clostridium difficile</em> selective medium
3. Dermatophyte infection in the nails is isolated using potatoes dextrose agar
4. Dengue fever by immune response is isolated using minimal essential medium (MEM)
1. Dysentery caused by <em>Shigella dysenteriae</em>
- Collection of fecal specimens and poultry (100) fecal samples collected from were processed according to published protocols.
- Streak one loopful of fecal sample on MacConkey Lactose Agar (MLA) and Xylose Lysine agar
- Incubated at 37 C for 24 hours
- The MLA plates showing the presence of convex and colorless colonies are considered for further identification.
- Similarly, the XLD plates showing appearance of presence of translucent or red colored colonies are taken for further identification.
2. Antibiotic associated diarrhea caused by <em>Clostridium difficile </em>(detection of toxin)
- Mix equal parts of industrial absolute alcohol and the fecal specimen.
- Homogenize using a vortex mixer.
- Leave at room temperature for 1 hr.
- Inoculate on <em> </em><em>Clostridium difficile </em>Selective Agar
- Incubate at 37ºC in a conventional anaerobic gas jar
- Observe after 24-48 hours in anaerobic environment .
- Use gram stain reagent and Malachite green for morphological study.
- Observe positive bacillus cell shape with single or chains(2-6)cell and sub terminal endo spore forming after gram staining
3. Dermatophyte infection in the nails
- Prepare potatoes dextrose agar (PDA)
- Collect clippings from the nail with a sterile scalpel after carefully washing the site with 70% alcohol.
- Dissolved in two drops of 10% KOH on a glass slide, if the sample is thick, use 20% KOH
- Heat the slide few seconds over a spirit lamp, do not allow to boil.
- Observe preparation under a low power of the microscope in reduced light.
- Look out for presence of mycelia fragments and the distribution of spores inside.
4. Dengue fever by immune response
- Dilute blood samples in minimal essential medium (MEM) containing 2% fetal bovine serum (FBS)
- Add diluent to a cultured monolayer of C6/36 cells for 1 hour with gentle rocking in a 28 °C CO2 incubator.
- After 1 hour, remove the inoculum and cells
- Culture for 7 days in MEM with 2% FBS and antibiotics.
- Collect culture supernatant on day 7 and infect a new batch of C6/36 cells
- Repeat the procedure three times to achieve passage 3 (P3) isolates.
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