In molecular biology, restriction fragment length polymorphism, orRFLP, is a technique that exploits variations in homologous DNA sequences. It refers to a differencebetween samples of homologous DNAmolecules from differing locations ofrestriction enzyme sites, and to a related laboratory technique by which these segments can be illustrated. InRFLP analysis, the DNA sample is broken into pieces and (digested) byrestriction enzymes and the resultingrestriction fragments are separated according to their lengths by gel electrophoresis. Although now largely obsolete due to the rise of inexpensive DNA sequencing technologies, RFLP analysis was the first DNA profilingtechnique inexpensive enough to see widespread application. RFLP analysis was an important tool in genome mapping, localization of genes forgenetic disorders, determination of riskfor disease, and paternity testing.
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I believe that what is meant with the phrase "older explanation" is none other that superstition. Therefore, I will give you one superstitious belief which is now explained by science.
In Southeast Asia, people used to believe that St. Elmo's fire appears in remote areas because a bloody battle occured in that place leaving disembodied souls of the dead wandering there in the night and even in sunshine!
For generations, many people believed that this is a fact and some even say that these apparitions are diabolical or demonic in nature. So often, caution is advised to people whenever a ball of fire appears out in the open.
However, recent breakthroughs in the 21st century helped scientists to explain this mysterious phenomena. They found out that St. Elmo's fire is actually plasma, nothing special. Now, plasma is considered one of the states of matter! But mind you, it still does not explain why St. Elmo's fire seems to have a mind of its own!
Enzyme activity can be affected by a variety of factors, such as temperature, pH, and concentration. Enzymes work best within specific temperature and pH ranges, and sub-optimal conditions can cause an enzyme to lose its ability to bind to a substrate.