Answer:2,1,3,5,4
Explanation:
Transgene are artificially introduce gene in an organism.
Transgenic organism- they are organism that contains transgene. Transgene and Transgenic organism are terms used in genetic engineering. Gene cloning or transgene could lead to transformation of individual and have been used in producing Genetically modified organisms.
The steps include;
1.Cut out the insulin gene using restriction enzymes. Cut the bacterial plasmid with the same restriction enzymes.- restriction enzymes are endonucleases
2. Ligase -pastes the insulin gene into the bacterial plasmid.
3. The bacterium multiplies, producing many bacterial cells that make the insulin protein
4.The recombinant plasmid containing the Insulin gene is taken up by a bacterium.
5.. Human insulin is isolated from the transgenic bacteria
Note that for gene cloning - there must be a gene to be introduced, a vector, host cell where the gene will multiply and a means of introduction that transformant the organisms and a means of getting a whole organism.
the air pressure will also increase
Answer:
it would affect by diseases and deaths of no food
Explanation:
