Answer:
C.The added epitope disrupts the function of the tagged protein
Explanation:
When an additional sequence is tagged to a protein to use comercial antibodies, there are several reasons why this procedure wouldn't work as expected (note that we're assuming the protein is being expressed but it's not possible to detect it).
For example, the sequence of nucleotide added to codify for the tagged epitope are removed during the RNA processing. In that case, the protein would be expressed without the epitope, so it would be impossible to localize it with the antibodies.
Also, it could be that the new epitope is affecting some way the protein folding, making it not functional. This way, it would be degraded by the cell so it wouldn't be detected.
Another possibility is that the epitope doesn't affects the protein folding nor its function, but during the folding ends up in a conformation that makes it inaccesible for the antibody.
In summary, the way as the possible answers to this question are shown, the correct option seems to be C:<em> The added epitope disrupts the function of the target protein.</em>
The only exception would be nuclear energy. Heavy elements like Uranium got their stored energy from ancient supernovae, that doesn't come from the sun. And fusing hydrogen is tapping energy that has been around since the Big Bang.
Hope this helped :)
Correct answer: A). Gathering evidence
The scientific explanation uses measurements and observations in order to explain something in the natural world. The Scientist uses the scientific method to collect evidence in the experiment that is related to the hypothesis.
They can gather the evidence by just observing the natural world and its phenomena or by performing an experiment in the laboratory or by running a model.
Hence, the correct answer would be option A.
Chlorophyll is a green pigment found in plants. Plants use chlorophyll and light to make food.
