I think the correct stage would be In the prophase.
Answer:
The correct answers are 2.23 * 10^8 CFU/ml and 2 colonies.
Explanation:
Based on the given information, 0.1 ml is the amount of bacterial culture plated, 10^-5 is the dilution factor and the number of bacterial colonies produced is 223.
A) 223 is the number of colonies produced when 0.1 ml of the culture is plated. Therefore, the number of colonies produced when 1 milliliter of bacterial culture plated us (223/0.1)*1 = 2230
The calculation of the CFU/ml is done by using the formula,
CFU/ml = Number of colonies per ml plated / dilution factor
Thus, 2230/10^-5
= 2230 * 10^5 or 2.23 * 10^8 CFU/ml
B) The number of colonies, which would grow on a plate, which is inoculated with 0.1 ml volume of 10^-7 dilution from the similar bacterial stock will be calculated as,
CFU/ml = Number of colonies per ml plated/ dilution * volume plated.
2.23 * 10^8 CFU/ml = Number of colonies per ml plated / 10^-7 * 0.1
Number of colonies per ml plated = 2.23 * 10^8 * 0.1 / 10^7 = 2.23 or 2 colonies.
I don’t know 22 but I think 21 is B
