Answer:
Loss of cell wall integrity or
Explanation:
Grams staining i a staining method used to classify bacteria into two main classes. Gram positive and Gram negative.
They are differentiated according to the ability of their cell wall to retain the primary stain color of the crystal violet stain or the counter stain pink.
Bacillus subtilis is a Gram positive bacteria hence it is supposed to stain purple.
Therefore, when it stained pink is an error. it shows loss of the cell wall integrity due to old age, overdecolorization or over fixing with heat.
Answer: C. Control Question
