The buffer was too acidic i.e when a blood film is viewed through the microscope, the RBCs appear redder than normal, the neutrophils are barely visible, and the eosinophils are bright orange.
<h3>What are buffer used for?</h3>
Buffers are employed to keep the pH of a solution steady because they can neutralize little amounts of extra acid or base. There is a specific pH range for a certain buffer solution and a predetermined amount of acid or base that can be neutralized before the pH changes. The buffer capacity is the maximum amount of acid or base that can be supplied to a buffer without causing a pH change.
As a pH value greater than 7.8 or lower than 6.8 might result in mortality, human blood contains a buffer of carbonic acid (H2CO3) and bicarbonate anion (HCO3-) to keep blood pH between 7.35 and 7.45.
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