Question

When a blood film is viewed through the microscope, the RBCs appear redder than normal, the neutrophils are barely visible, and the eosinophils are bright orange. What is the most likely cause

Answer 1

The buffer was too acidic i.e when a blood film is viewed through the microscope, the RBCs appear redder than normal, the neutrophils are barely visible, and the eosinophils are bright orange.

What are buffer used for?

Buffers are employed to keep the pH of a solution steady because they can neutralize little amounts of extra acid or base. There is a specific pH range for a certain buffer solution and a predetermined amount of acid or base that can be neutralized before the pH changes. The buffer capacity is the maximum amount of acid or base that can be supplied to a buffer without causing a pH change.

As a pH value greater than 7.8 or lower than 6.8 might result in mortality, human blood contains a buffer of carbonic acid (H2CO3) and bicarbonate anion (HCO3-) to keep blood pH between 7.35 and 7.45.

Learn more about buffer here:

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