Question

Replacing native gel electrophoresis with nonreducing sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) during the purification step results in eluates with no enzymatic activity. The best explanation of this observation would be:

Answer 1

Replacing native gel electrophoresis with SDS results in eluates with no enzymatic activity because it confers a negative charge to proteins, thereby neutralizing them.

What is sodium dodecyl sulfate?

The sodium dodecyl sulfate (SDS) is a detergent used in molecular biology laboratories.

This detergent (SDS) confers a negative charge to proteins, thereby neutralizing them (loss of their enzymatic activity).

In conclusion, SDS results in eluates with no enzymatic activity because this detergent may neutralize them.

Learn more about SDS detergent here:

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