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kondor19780726 [428]
2 years ago
13

Replacing native gel electrophoresis with nonreducing sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) dur

ing the purification step results in eluates with no enzymatic activity. The best explanation of this observation would be:
Biology
1 answer:
Grace [21]2 years ago
6 0

Replacing native gel electrophoresis with SDS results in eluates with no enzymatic activity because it confers a negative charge to proteins, thereby neutralizing them.

<h3>What is sodium dodecyl sulfate?</h3>

The sodium dodecyl sulfate (SDS) is a detergent used in molecular biology laboratories.

This detergent (SDS) confers a negative charge to proteins, thereby neutralizing them (loss of their enzymatic activity).

In conclusion, SDS results in eluates with no enzymatic activity because this detergent may neutralize them.

Learn more about SDS detergent here:

brainly.com/question/14668619

#SPJ1

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Vlad1618 [11]

Answer:

Trophe means food (A)

Explanation:

Trophe is a Greek word for food

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4 years ago
The sole of a gecko's foot is covered with millions and millions of small, dry "hairs" that make direct contact with surfaces, a
tamaranim1 [39]

Answer: Van der Waals forces

Explanation:

Van der Waals forces are weak intermolecular forces that depend on the distance between two particles. They are caused by correlations in the change in polarization between two nearby particles. To put it in other words, when a particle changes its polarization (becomes more positive on one end and more negative on the other), so does the adjacent particle, and the next one, and so on. This causes these particles to stick together weakly.

The tiny "hairs" increase the surface area of the gecko's feet in contact with the wall, which makes the bond stronger and allows it to support all of its weight.

Because experiments have shown that geckos stick well to both hydrophobic and hydrophilic surfaces, we can assume there aren't any hydrogen bonds present.

Ionic bonds can't be present either because geckos wouldn't stick to electrically neutral surfaces, as these bonds require charged molecules.

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3 years ago
What was Rudolph Virchow’s contribution to the cell theory?
Ratling [72]
Virchow's is credited with very several important discoveries. 
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4 years ago
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Darya [45]

\huge\bold\pink{answer}

  1. <em><u>Nail Cutter</u></em>
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  3. <em><u>Cuticle Nipper</u></em>
  4. <em><u>NipperNail Buffer </u></em>
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3 years ago
How is efficiency related to systems?
WARRIOR [948]
The efficiency indicates the manner in which the inputs are used by the system. Being efficient means the system uses inputs in a `right' way. If the input-output ratio is adverse, we say that the system is inefficient though it produces the desired output.
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