Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Igneous rock.
Igneous rock forms from cooled and hardened magma or lava.
Staphylococcus aureus is a gram-positive bacteria that cause a wide variety of clinical diseases. Infections caused by this pathogen are common both in community-acquired and hospital-acquired settings. The treatment remains challenging due to the emergence of multi-drug resistant strains such as MRSA (Methicillin-Resistant Staphylococcus aureus). S. aureus does not normally cause infection on healthy skin, however, if it is allowed to enter the internal tissues or bloodstream, these bacteria may cause a variety of potentially serious infections. This activity describes the evaluation and treatment of Staphylococcus infections and reviews the role of the interprofessional team in managing patients with these diseases.
Some of the core reasons are:
1) Deforestation
2) Excessive use of fossil fuels
3) Unevenness of Garbage
4) Increasing Population
5) Expansion of Urban area
Hope this helps!
the answer is algae because algae is an aquatic plant
