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bonufazy [111]
2 years ago
12

Rapid lateral flow assays based on the quantification of magnetic nanoparticle labels for multiplexed immunodetection of small m

olecules: application to the determination of drugs of abuse
Biology
1 answer:
PolarNik [594]2 years ago
6 0

A rapid lateral flow immunoassay is presented that uses carboxyl-modified superparamagnetic nanoparticles as labels that can be quantified by highly sensitive multi-channel electronic readers. The approach is generic in that it is likely to be applicable to numerous small molecules. The method permits both single- and multiplex assays at a point-of-need without sample pretreatment. It is user-friendly and offers attractive characteristics demonstrated here for detection of morphine, fentanyl and methamphetamine in . The competitive immunoassay uses commercially available reagents that do not require special permissions. After migration of sample, the lateral flow test strips are subjected to an alternating magnetic field at two frequencies. The response from the nanolabels is readout at a combinatorial frequency from the entire volume of a porous immunochromatographic membrane by the magnetic particle quantification technique.

application to the determination of drugs of abuse.

Estimating the detection time of a drug in urine is complex because of many different influencing factors and the lack of experimental data. Detection times vary depending on dose and route of administration, metabolism and characteristics of the screening and confirmation assays. Using a cut-off value of 1000 ng/mL, urinary samples can be positive for amphetamine for up to 5 days after intake of the drug.

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Chromosomes and DNA

Chromosomes are DNA wrapped around proteins to form an X-shaped structure.



The diagram will help you see the relationship.

1. Chromosomes are found in the nucleus
2. Chromosomes are made of DNA
3. Sections of chromosomes are called genes



DNA - deoxyribonucleic acid (it is the genetic code that contains all the information needed to build and maintain an organism)





Chromosome Structure







Chromosome Numbers

Each organism has a distinct number of chromosomes, in humans, every cell contains 46 chromosomes. Other organisms have different numbers, for instance, a dog has 78 chromosomes per cell.

Somatic Cells - body cells, such as muscle, skin, blood ...etc. These cells contain a complete set of chromosomes (46 in humans) and are called DIPLOID.

Sex Cells - also known as gametes. These cells contain half the number of chromosomes as body cells and are called HAPLOID

Chromosomes come in pairs, called Homologous Pairs (or homologs). Imagine homologs as a matching set, but they are not exacly alike, like a pair of shoes.

Diploid cells have 23 homologous pairs = total of 46

Haploid cells have 23 chromosomes (that are not paired) = total of 23

Homologous Chromosomes



Sex Determination

Chromosomes determine the sex of an offspring. In humans, a pair of chromosomes called SEX CHROMOSOMES determine the sex.

If you have XX sex chromosomes - you are female

If you have XY sex chromosomes - you are male

During fertilization, sperm cells will either contain an X or a Y chromosome (in addition to 22 other chromosomes - total of 23). If a sperm containing an X chromosome fertilizes an egg, the offspring will be female. If a sperm cell containing a Y chromosome fertilizes an egg, the offspring will be male.

Creation of a Zygote

When two sex cells, or gametes come together, the resulting fertilized egg is called a ZYGOTE

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Karyotype

A karyotype is a picture of a person's (or fetus) chromosomes. A karyotype is often done to determine if the offspring has the correct number of chromosomes. An incorrect number of chromosomes indicates that the child will have a condition, like Down Syndrome

Compare the Karyotypes below













Notice that a person with Down Syndrome has an extra chromosome #21. Instead of a pair, this person has 3 chromosomes - a condition called TRISOMY (tri = three)

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Other conditions result from having the wrong number of chromosomes:

Klinefelters Syndrome - XXY (sex chromosomes)

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Explanation:

hope this helps

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