Question

A. Watch the video B. Answer questions below 0:00 5:05 This In The Clinic video will task you 1x O 1.Briefly explain why S. salivarius was selected 2.Briefly explain why the S. salivarius can not be cultured 3. Briefly explain steps how PCR works 4. In the crime scene, which specimen was used for PCR testing? 5. Explain why PCR testing is used in this crime scene 6. What is the purpose of gel-electrophoresis? 7. Briefly explain steps how gel electrophoresis works. 8. Briefly explain how the case was solved. S​

Answer 1

The S. salivarius is chosen because it the predominant gram positive bacteria that colonizes the oral cavity shortly after birth.

What is PCR?

Polymerase chain reaction(PCR) test is a type of test that is used to identify a particular deoxyribonucleic acid of an organism.

Streptococcus salivarius cannot be cultured especially when the test sample is contaminated with other body normal flora.

The steps of PCR testing include the following:

• Denaturation by heat

• Annealing Primer to Target Sequence

• Extension

• End of the First PCR Cycle

On a crime scene, the specimen that can be used for PCR testing are blood and body secretions.

PCR testing can be done on a scene with blood spills.

Learn more about gel electrophoresis here:

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