An opaque object is any object that is not transparent (allowing all light to pass through it), or translucent (allowing some light to pass through it.) Knowing this we can auto-matically dismiss option D),B), and C) because these all let some form of light through them so our answer would then be A) because light does not pass through wood like it would the others.
Answer:
C.The added epitope disrupts the function of the tagged protein
Explanation:
When an additional sequence is tagged to a protein to use comercial antibodies, there are several reasons why this procedure wouldn't work as expected (note that we're assuming the protein is being expressed but it's not possible to detect it).
For example, the sequence of nucleotide added to codify for the tagged epitope are removed during the RNA processing. In that case, the protein would be expressed without the epitope, so it would be impossible to localize it with the antibodies.
Also, it could be that the new epitope is affecting some way the protein folding, making it not functional. This way, it would be degraded by the cell so it wouldn't be detected.
Another possibility is that the epitope doesn't affects the protein folding nor its function, but during the folding ends up in a conformation that makes it inaccesible for the antibody.
In summary, the way as the possible answers to this question are shown, the correct option seems to be C:<em> The added epitope disrupts the function of the target protein.</em>
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I’n pretty sure the answer is supposed to be about the tectonic plates. The plates constantly shift the land creating holes/dips in the earth and mountains in other places.
