Question

We used the enzyme, taq polymerase, which is derived from the thermus aquaticus bacterium, in our pcr procedure. Why is this enzyme important for efficiency in pcr?.

Answer 1

The use of a thermophilic DNA polymerase such as Taq polymerase prevents the denaturation of the enzyme during the heating that is important to separate the newly synthesized strand. So using this enzyme simplifies the PCR technique and increases its efficiency.

Taq DNA polymerase is highly efficient, when it reaches the optimum temperature, it becomes fully functional. It also has a half-life of over two hours (at 92°C), high amplification capacity, and it can add upto 150 nucleotides per second.  It is "special" enzyme because it comes from the bacterium Thermus aquaticus, which lives in hot springs. It is therefore thermostable even at high temperatures, while other polymerases (e.g. E. coli) are not.

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