Question

We used the enzyme, taq polymerase, which is derived from the thermus aquaticus bacterium, in our pcr procedure. Why is this enzyme important for efficiency in pcr?.

Answer 1

The enzyme Taq polymerase is required in the PCR process because it is the main enzyme that synthesizes the new DNA strands complementary to the template strand and without the activity of this enzyme the PCR will be useless and it cannot make the copy of the given strand in any way. Thus, this enzyme is very much necessary for the efficient working of the PCR.

Polymerase Chain Reaction is a very efficient technique that can able to detect and copy even a small amount of DNA by performing a set of reactions at different temperatures.

The PCR process begins with the denaturing of the template strand at 95°C, followed by annealing in which the appropriate primers bind to the single strands of DNA at 50-56°C, followed by extension at 72°C in which the thermostable Taq polymerase synthesizes the strand complementary to the template DNA.

Learn more about PCR here

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