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Varvara68 [4.7K]
2 years ago
14

Your research partner has centrifuged the cell extract at 10,000g for 10 minutes, but is not sure which fraction (pellet or supe

rnatant) contains mdh. what should you tell your research partner?
Social Studies
1 answer:
oksano4ka [1.4K]2 years ago
4 0

Both the pellet (mitochondrial MDH) and the supernatant (cytoplasmic MDH) contain MDH.

Separation has been achieved. One does not need to separate each fraction to separate two cell fractions from each other.

Adding additional steps before and after the mitochondrial step will increase the purity, but based on the description to isolate the two enzymes, the job is done. Tell your partner to keep both fractions.

A compact, sterile dosage form with an ovoid or rod shape that is essentially pure steroid hormones compressed; it is designed for subcutaneous implantation in bodily tissues and acts as a depot for the hormone's prolonged, gradual release.

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