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Drupady [299]
1 year ago
7

When testing a four-wheel drive electronic component, the technician will use a scan tool to pull codes, and then a ________ to

diagnose any codes found.
Biology
1 answer:
Sergeeva-Olga [200]1 year ago
3 0

Answer: Aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa

Explanation:aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa

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The CRISPR/Cas9 system can cleave genomic DNA at sequences other than the desired target, a phenomenon referred to as off target
Deffense [45]

Answer:

The minimum length of a sgRNA sequence to avoid off target cleavage by the CRISPR/Cas system in the fly fruit genome is 14 bases

Explanation:

We are trying to use the CRISPR/Cas system to cleavage the genome of the fruit fly (which is 1.4x10^8 bp long). Also we desire the cleavage to be unique. That means we need a target sequence long enough to be able to assume it will only appear once in the genome.

First, we should think that in every position, we can find one out of four different nucleotide (A, C, T, G). So, the probability of getting a sequence of a given length "n" will be (1/4)^n (We are assuming that the probability of finding a nucleotide in the position "i", it's independent of the nucleotide we find in any other position "j").

Also, to know how many times a sequence will appear in a genome (the expected value of occurrence), we must multiply the probability of that sequence to randomly occur by the length of the genome. For our specific example, the number of occurence of a sequence of length "n" is:

nºoccurence=[(1/4)^n]*1.4*10^8

But in this case, what we want is the expected number of times the sequence will appear to be 1, and we want to obtain the length of the target sequence (n).

Given the information above, we know that:

[(1/4)^n]*1.4*10^8 =1

[(1/4)^n]=(1/1.4*10^8)=1.4*10^-8

Then, if we want to calculate n, we can use logarithms and its properties to get:

log[(1/4)^n]=log[1.4*10^-8]

n*log[(1/4)]=log[1.4*10^-8]

n=log[1.4*10^-8]/log[(1/4)] => n=13.29 approximately.

As the sequence needs to have a natural number of elements, <u>we can conclude that using a target sequence of a minimum of 14 bases with the CRISPR/Cas system in the fly fruit genome should be enough to avoid off target cleavage.</u>

3 0
3 years ago
Many soil-dwelling bacteria produce molecules that can be naturally synthesized and kill other species of bacteria. What are the
Masteriza [31]

Many soil-dwelling bacteria produce molecules that can be naturally synthesized and kill other species of bacteria. What are these bacteria-killing molecules called antibiotics

Antibiotics are effective pills which can be usually safe. Although they are very helpful in fighting disease,  antibiotics can actually be harmful. Important Facts About Antibiotic Safety: Antibiotics can cause side effects such as allergic reactions and severe and potentially life-threatening diarrhea caused by the bacterium Clostridium difficile (C. diff).

Antibiotics can also interfere with other medications you may be taking. Antibiotic side effects account for nearly one-fifth of  emergency room visits. These conditions are the most common reasons for emergency room visits for children under the age of 18. Taking antibiotics you don't need unnecessarily exposes you  to the drug's side effects and denies you the benefits.

Learn more about antibiotic here:

brainly.com/question/6970037

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5 0
2 years ago
Read 2 more answers
What are some synonyms to mitigate?​
erastova [34]

Answer: llay, alleviate, assuage, ease, help, mollify, palliate, relieve, soothe. Words Related to mitigate. abate, lighten, moderate, soften, temper. cure, heal, remedy. amend, correct, emend, fix, mend, rectify, reform, repair.

Explanation: hope this helps

6 0
3 years ago
How is water necessary for the structure of a green plant?
ch4aika [34]
Like all life on earth, plants need water to survive and grow. Indeed, like humans, water is the primary element that makes up the structure of plants. Human bodies are comprised of around 70 percent water, but in plants this proportion can be as much as 95 percent. Water is also essential to the way a plant receives nutrients and provides energy for itself. Thus, water is arguably the most essential substance required by plants. That is why many elements of permaculture design – from using swales and contouring to slow runoff from the land and allowing it to sink into the soil to mulching to prevent evaporation of moisture from the soil surface – emphasis the preservation of water so that it can be provided to plants.

hope this helped :)
alisa202
6 0
3 years ago
The parathyroids are responsible for regulating proper levels of which elements in the blood?
blondinia [14]
Phosphate and calcium
3 0
3 years ago
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