Question

Why might you not see as much bacterial colonization of microbes from human saliva on plates cultured at 25oc versus plates incubated at 37oc?

Answer 1

Bacteria need an optimum temperature for their growth, so this is the reason why we might not see as much bacterial colonization of microbes from human saliva on plates cultured at 25 °C versus plates incubated at 37 °C.

Why do bacteria only grow at certain temperatures?

• The main justification for incubating bacterial cultures at various temperatures is that different temperatures favor the growth of particular bacteria.
• Most infections and typical ambient bacteria are classified as mesophiles, which thrive in temperatures between 20 and 40 degrees Celsius.
• An optimum temperature is required for the enzymes to show their activity, and for the division also many enzymes are involved in the process. So, the bacterial culture can be seen at a particular incubation temperature only.

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