The theory of spontaneous generation comes from the idea that living organisms can arise from even other places, instead of eggs, seeds or even reproduction. The contrary was thought in the ancient times.
Louis Pasteur agreed with the beliefs of the ancient times and supported the idea that cells come from pre-existing cells. He conducted the S-shaped tube experiment to prove the same.
In this experiment, he took an S-shaped flask and a regular flask, and boiled proteins and liquid with other nutrients, to kill the microorganisms present in it. In the normal flask, liquid showed changes when exposed to air, but not in the s-shaped flask.
When he removed the s-neck, it is then that the liquid changed, proving that it wasn't the air affecting the change, but the microorganisms present in it. Thus, these microorganisms did not spontaneously generate.
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