Question

roposed that the 32P label was entering PE molecules by direct exchange (swapping phosphate groups with those found in solution) and NOT through synthesis of new PE by bacterial cells. What experimental modification can show this is NOT the case

Answer 1

This can be demonstrated by using an acellular environment to observe the 32P label entering phosphatidylethanolamine (PE) molecules by direct exchange.

Phosphatidylethanolamine (PE) is a major lipid component of cellular membranes in a wide range of organisms.

A scientist proposed that the 32P label was entering PE molecules by direct exchange, this can be proved using acellular environment.

In acellular environment, there aren't any bacterial cells to synthesize new PE. So if the 32P is incorporated into the PE in the acellular environment that means the proposed idea in the question stem is correct.

If no 32P is incorporated into PE in the acellular environment, then that means proposed idea is not correct.

Disclaimer: The question is not completed.

A scientist proposed that the 32P label was entering PE molecules by direct exchange (swapping phosphate groups with those found in solution) and NOT through synthesis of new PE by bacterial cells. What experimental modification can show this is NOT the case?

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