Question

When 15n-labeled aspartate is fed to animals, the 15n-label rapidly appears in many amino acids. What reactions are occurring to allow this to happen?

Answer 1

Aspartate + a- keto acid >> Oxaloacetate + a amino acid

This reaction is occur because the a- keto acid in the equation can come from any amino acid, the 15 N from aspartate is rapidly transferred to other amino acids.

15N is symbol use as the isotope of nitrogen with mass number 15. comprises of 0.4 % of stable nitrogen found, so the relative abundance of each in amino acid is simply a reflection of how much is found in nature in general.

This isotopes is enrichment of the labelled amino acids was determined following a previously developed procedure comprising by determination of the spectral purity of the selected natural abundance amino acids.

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