Question

What would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator?

Answer 1

In the absence of tryptophan, the genes trpA-E will not be transcribed.

If there were a mutation in the operator region of the trp operon, there would be a nucleotide change in the sequence of the operator. Mutations in the repressor that prevent its binding to the operator will lead to constitutive expression Mutations that prevent binding of the inducer without affecting the ability to bind to the operator lead to a non‑inducible phenotype.

The tryptophan binds to the trp repressor and causes it to change shape, converting into its active form. The trp repressor with the bound tryptophan attaches to the operator, blocking RNA polymerase from binding to the promoter and preventing transcription of the operon.

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