If one wishes to clone a gene using typical restriction endonucleases, how does the restriction endonuclease identify the appropriate cut sites in the genome using the palindromic sequence.
Restriction endonucleases are a family of enzymes usually found in bacteria that cuts DNA at a specific sequence called recognition site. There are three type of restriction endonuclease Type I, Type II, and Type III.
Each endonuclease recognizes a particular sequence called palindromic sequence which is a sequence that reads the same sequence in both the strands either reading from the 3 prime end or 5 prime end.
The endonuclease cuts the palindromic sequence in such a way that it produces sticky ends.
To learn more about restriction endonucleases here
