Glucose is the preferable source of energy for E. coli cells. By adding small amounts of glucose, 0.05% in this case, it would enable cell growth to proceed as usual until it has reached mid log growth phase. At this point, the small amount of glucose added should have been depleted and the E.coli cell will now be dependent on lactose present as its source of nutrients. Lactose will also inactivate lac repressors and given the lack of glucose, CAP binding to high amounts of cAMP will be activated and increase expression of lac operon genes.
It should be noted that this system of expression may not tightly regulate the expression of lac operon before the E.Coli reaches exponential phase. As lactose is still present, lac repressors will be inactivated throughout the entire experiment and hence small amounts of proteins might be produced even when not auto-induced. After auto-induction, CAP-cAMP protein complex will simply upregulate expression of lac operon genes.
Would if i cpuld see the questions
<span>When river water is no longer flowing downhill, the water slows down. This makes sediment drop to the bottom. Sediment deposited where a river flows into an ocean or lake builds up and a landform called a delta is formed.</span>
Answer:
I think that it is missence mutation
Answer: Elephants
Explanation:
Elephants have being able to survive in the savannah due to its ability to adapt. Elephants natural have no sweet glands so they have to device other means to cool their selfs, th ey achieve this by flapping their ears to create the cooling effects they need to survive in their environment. They also posses tusk and long trunks which they used for eating and drinking. The adaptation of the elephants to the African savannah has being due to the effects of environment and the genes. A popular example is an African elephant is different from it’s counterpart from Asia. Africa elephants have bigger ears unlike Asians which are smaller