Question

Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of a. post-transcriptional processing. b. post-translational processing. c. nucleic acid hybridization. d. restriction fragment ligation.

Answer 1

The use of mRNA and reverse transcriptase in this case is part of a strategy to solve the problem of post-transcriptional processing. So the correct option is A.

What is post-transcriptional processing?

The process of transcription results in the formation of a heterologous nuclear RNA (hnRNA) or a precursor RNA (PreRNA). This undergoes post-transcriptional processing to form mRNA. Following are the steps -

1. Splicing - it removes the non-coding introns so that only coding regions called exons remain.
2. Capping - a 5'-methylguanosine triphosphate is attached at the 5' end of the RNA.
3. Tailing - About 200 polyadenylate residues attach at the 3' end of the RNA.

These processes result in the formation of mRNA.

Therefore the correct option is A.

Read more about RNA processing, here

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