Reverse transcriptase is used in the lab to make complementary DNA (cDNA), which is complementary to a specific mRNA.
A reverse transcriptase is a laboratory tool used to create complementary DNA (cDNA), which is identical to a certain mRNA. In 1970, the RNA-dependent DNA polymerase known as reverse transcriptase was found in various retroviruses, including the human immunodeficiency virus (HIV) and the avian myeloblastosis virus (AMV).
The reverse transcriptase is a very helpful tool for molecular biology research since it catalyzes the transformation of RNA template molecules into a DNA double helix. Reverse transcriptases are frequently used to create complementary DNA (cDNA) libraries from a variety of expressed mRNAs. When paired with the polymerase chain reaction method (RT-PCR), reverse transcriptases are also used to assess the degree of mRNA production. Three enzymatic processes are present in reverse transcriptase: RNA-dependent DNA polymerase, RNase H, and DNA-dependent DNA polymerase.
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