Question

• bahrami, m. et al. deep feature extraction of single-cell transcriptomes by generative adversarial network. bioinformatics (oxford, england) 3, 346 (2020)

Answer 1

Single-cell RNA-sequencing (scRNA-seq) provides the chance to analyze heterogeneous cellular compositions and probe the patterns of gene expression that are unique to each cell type under various circumstances. However, batch effects like lab setups and individual variability make it difficult to use them in cross-condition designs.

What is Single-cell transcriptomes ?

In single-cell transcriptomics, the messenger RNA levels of hundreds to thousands of genes are simultaneously measured to assess the degree of gene expression in individual cells within a particular population.

Advantages :

• Integrated protocol proceeds directly from whole cells and preserves sample integrity.

• High resolution analysis enables discovery of cellular differences typically hidden by bulk sampling methods.

• Robust transcriptome analysis down to single-cell input levels for high-quality samples.

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