Genetic modification of micro-organisms is conducive to the selection of high production producers of high-value-added chemicals, but a deficient of genetic tools limits the industrialization of most wild species
It is very important to develop host-independent gene editing tools which can be used for genetic modification deprived strains.
A easily found Cas12k-based genetic engineering toolkit (C12KGET) that can result into genomic integration of small parts up to 10 kb in size with up to 100% efficiency in challenging strains.
By using C12KGET, we accomplished the first example of highly efficient genome editing in Sinorhizobium meliloti, which was successfully solved the question of that industrial strains are difficult to genetically modify, and increased vitamin B12 production by 25%.
Cas12k can be directly used for transcriptional regulation of genes with up to 92% efficiency due to its naturally inactivated nuclease domain
